- Aladar ( Dinosaur 2000 ) as Basil
- Kron ( Dinosaur 2000 ) as Professor Ratigan
- Carla ( Starfall ) as Major Dr. David Q. Dawson
- Elmo ( Sesame Street ) as Olivia Flaversham
- Otis (Barnyard ) as Fidget
- Pig ( Barnyard ) as Toby
- Big Bird ( Sesame Street ) as Hiram Flaversham
- Fix It Felix ( Wreck it Ralph ) as Mrs. Judson
- Velma ( Scooby Doo ) as Queen Mousetoria
- Pot Billed Pig as Miss Kitty Mouse
- Crocodile as Bartholomew
- Mouse as Sherlock Holmes
- Owl as Dr. Watson
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